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Mouse TNF alpha PicoKine ELISA Kit

2020/4/3 16:38:08 瀏覽:
  • 【品牌】: 博士德
  • 【產(chǎn)品貨號(hào)】: EK0527
  • 【價(jià)格】: 1200  元
  • 【規(guī)格】: 48T/96T
  • 【貨期】: 現(xiàn)貨
  • 在線訂購
產(chǎn)品介紹
 產(chǎn)品說明
【品牌】: 博士德
【產(chǎn)品貨號(hào)】: EK0527
【價(jià)格】: 1200  元
【規(guī)格】: 48T/96T
【貨期】: 現(xiàn)貨
【儲(chǔ)存溫度】: -20

Tumor necrosis factor-alpha(TNF-alpha, or TNF) is secreted by macrophages in response to inflammation, infection and cancer. Human Tumor Necrosis Factor(TNF) and Lymphotoxin(TNF-beta) are cytotoxic proteins which have similar biological activities and share 30% amino acid homology. TNF-alpha is produced by monocytes, which can stimulate endothelial cells to produce the multilineage growth factor granulocyte-macrophage colony-stimulating factor and extend the role of this immunoregulatory protein to the regulation of hematopoiesis in vitro. TNF is a soluble protein that causes damage to tumor cells but has no effect on normal cells. Human TNF has been purified to apparent homogeneity as a 17.3-kilodalton protein from HL-60 leukemia cells and has showed cytotoxic and cytostatic activities against various human tumor cell lines. The human TNF cDNA is 1585 base pairs in length and encodes a protein of 233 amino acids. The mature protein begins at residue 77, leaving a long leader sequence of 76 amino acids. TNF-alpha has been mapped to human chromosome 6.

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每孔添加100ul樣品和標(biāo)準(zhǔn)品,37℃反應(yīng)90分鐘,不洗。

每孔添加100ul生物素標(biāo)記抗體,37℃反應(yīng)60分鐘。

0.01M TBS洗滌3次。

每孔添加100ulABC,37℃反應(yīng)30分鐘。

0.01M TBS洗滌5次。

7 TMB37℃避光反應(yīng)30分鐘以內(nèi)。

加入TMB終止液,讀數(shù)。


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